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Journal
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Cancer Research
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建立和表征三个抗阿法替尼的肺腺癌PC-9细胞系与增加剂量的阿法替尼开发
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Establishment and Characterization of Three Afatinib-resistant Lung Adenocarcinoma PC-9 Cell Lines Developed with Increasing Doses of Afatinib
Please note that all translations are automatically generated.
Click here for the English version.
建立和表征三个抗阿法替尼的肺腺癌PC-9细胞系与增加剂量的阿法替尼开发
DOI:
10.3791/59473-v
•
09:38 min
•
June 26, 2019
•
Toshimitsu Yamaoka
,
Motoi Ohba
,
Yuki Matsunaga
,
Junji Tsurutani
,
Tohru Ohmori
1
Advanced Cancer Translational Research Institute
,
Showa University
,
2
Division of Breast Surgical Oncology, Department of Surgery
,
Showa University School of Medicine
,
3
Division of Allergology and Respiratory Medicine, Department of Medicine
,
Showa University School of Medicine
Chapters
00:04
Title
00:38
Initial Afatinib PC-9 Cell Exposure Determination by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) Assay
01:46
Continuous Afatinib Exposure
03:01
PC-9 Cell Growth Curve Determination
03:46
Epidermal Growth Factor Receptor (EGFR) Genomic DNA Alteration Evaluation
04:53
Epidermal Growth Factor Receptor (EGFR) Protein Alteration Evaluation
07:39
Results: Afatinib-resistant Cell Characterization
08:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
开发了一种从肺腺癌PC-9细胞中建立抗阿法替尼细胞系的方法,并对抗性细胞进行了特征描述。该耐药细胞可用于研究表皮生长因子受体酪氨酸激酶抑制剂抑制剂机制,适用于非小细胞肺癌患者。
Tags
Afatinib Resistance
Lung Adenocarcinoma
PC-9 Cell Lines
Acquired Resistance
Stepwise Dose Escalation
MTT Assay
Cell Culture
Subconfluency
Dose-dependent Resistance
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