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Immunology and Infection
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小鼠特拉切斯刷细胞的分离与定量评价
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Immunology and Infection
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Immunology and Infection
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
Please note that all translations are automatically generated.
Click here for the English version.
小鼠特拉切斯刷细胞的分离与定量评价
DOI:
10.3791/59496-v
•
10:25 min
•
June 12, 2019
•
Saltanat Ualiyeva
,
Eri Yoshimoto
,
Nora A. Barrett
,
Lora G. Bankova
1
Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital
,
Harvard Medical School
Chapters
00:04
Title
01:24
Preparation of Reagents
02:34
Dissection of Mouse Trachea
04:47
Tracheal Epithelial Digestion
08:31
Results: Analysis of the Isolated Brush Cells from Mouse Tracheas
09:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
刷细胞是罕见的胆碱能化学感觉上皮细胞发现在天真的小鼠气管。由于其数量有限,其功能在气道免疫和改造中的作用的体外评估具有挑战性。我们描述了一种通过流式细胞测定分离气管刷细胞的方法。
Tags
Tracheal Brush Cells
Epithelial Isolation
Papain Digestion
Choline Acetyltransferase
Fluorescent Reporter Mice
Transcriptional Analysis
RNA Sequencing
Upper Airway Epithelium
Dispase
DNase I
Tyrode’s Buffer
FACS Buffer
Euthanasia
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