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Isolering og kvantitativ vurdering af børste celler fra mus Tracheas
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
DOI:

10:25 min

June 12, 2019

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Chapters

  • 00:04Title
  • 01:24Preparation of Reagents
  • 02:34Dissection of Mouse Trachea
  • 04:47Tracheal Epithelial Digestion
  • 08:31Results: Analysis of the Isolated Brush Cells from Mouse Tracheas
  • 09:57Conclusion

Summary

Automatic Translation

Børste celler er sjældne kolinerge kemo sensoriske epiteliale celler findes i den naive mus luftrør. På grund af deres begrænsede antal, er ex vivo evaluering af deres funktionelle rolle i luftvejene immunitet og Remodeling udfordrende. Vi beskriver en metode til isolering af trakeal pensel celler ved flow cytometri.

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