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Bakteriecelle kultur på enkelt celleniveau inde i gigantiske vesikler
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles
DOI:

07:33 min

April 30, 2019

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Chapters

  • 00:04Title
  • 00:31Lipid-containing Oil Solution and Small Vesicle (SV) Preparation
  • 01:54Bacterial Cell Pre-culture
  • 02:45Giant Vesicle (GV) and Water-in-Oil (W/O) Bacterial Cell-containing Droplet Formation
  • 04:11Handmade Chamber and Supported Bilayer Membrane Preparation
  • 04:54GV Immobilization and Microscopic Bacterial Growth Observation
  • 06:04Results: Representative GV Containing Bacteria and Bacterial Growth Evaluation
  • 06:44Conclusion

Summary

Automatic Translation

Vi demonstrerer en enkelt cellekultur af bakterier inde i gigantiske vesikler (GVs). GVS indeholdende bakterieceller blev fremstillet ved dråbe overførselsmetoden og blev immobiliseret på en understøttet membran på et glas substrat for direkte observation af bakterievækst. Denne fremgangsmåde kan også tilpasses andre celler.

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