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在 Vivo 双色 2 光子成像中,基因标记报告器细胞在皮肤中
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
DOI:

05:45 min

July 11, 2019

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Chapters

  • 00:04Title
  • 00:55Imaging Setup
  • 02:12In Vivo Imaging
  • 04:07Results: Representative FITC-LPS Uptake
  • 05:03Conclusion

Summary

Automatic Translation

激活后免疫反应性成纤维细胞发生形态变化,促进细胞招募的改变。结合基因工程的纤维细胞特异性蛋白1(FSP1)-cre,利用2光子成像;tdTomato 絮凝停止絮凝(TB/TB)小鼠线和绿色荧光标记的脂多糖-FITC,我们可以说明在皮下成纤维细胞和体内形态变化中脂多糖的高度特异性吸收。

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