JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
使用膜内消化技术评估蛋白质与蛋白质相互作用
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
Please note that all translations are automatically generated.
Click here for the English version.
使用膜内消化技术评估蛋白质与蛋白质相互作用
DOI:
10.3791/59733-v
•
07:07 min
•
July 19, 2019
•
Takashi Obama*
1
,
Takuro Miyazaki*
2
,
Toshihiro Aiuchi
,
Akira Miyazaki
,
Hiroyuki Itabe
1
Division of Biological Chemistry, Department of Molecular Biology
,
Showa University School of Pharmacy
,
2
Department of Biochemistry
,
Showa University School of Medicine
Chapters
00:04
Title
00:43
Immunoprecipitation
02:42
On-membrane Digestion of Proteins
05:41
Results: GFP and Calpain-6 Associated Proteins
06:18
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们提出了一个方案,用于制备质谱样本的膜上消化技术。该技术有助于方便分析蛋白质与蛋白质的相互作用。
Tags
Protein-protein Interaction
On-membrane Digestion
Proteomic Analysis
Immunoprecipitation
Magnetic Beads
PVDF Membrane
Mass Spectrometry
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
对于拟肽与他们抗利什曼原虫活性混合赖氨酸型/精氨酸型单体及评价合成的有效方法
使用脚手架脂质体重构脂质近端蛋白质 - 蛋白质相互作用<em>体外</em
映射的适体对ATP的结合部位使用MicroScale程序热泳
时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态
原子力显微镜对单蛋白质分子的力谱
应用三色单分子焦虑研究蛋白质相互作用的相关性
膜蛋白对洗涤剂敏感相互作用的检测
原位
近接结扎法检测蛋白异构体的 Heterodimerization
通过 FASP 消化和数据独立分析,EPS-尿液的蛋白质配置文件
使用同质珠为基础的检测的查佩龙-科查佩龙相互作用研究
Read Article