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JoVE Journal Developmental Biology
Pan-myeloida differentiering av humant navelsträngsblod härrör CD34+ hematopoietiska Stem och progenitorceller
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Pan-myeloida differentiering av humant navelsträngsblod härrör CD34+ hematopoietiska Stem och progenitorceller

Article DOI: 10.3791/59836-v • 10:25 min • August 9th, 2019
August 9th, 2019 •
1, 1, 1
1Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona

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Här presenterar vi ett protokoll för immunophenotypic karakterisering och cytokin inducerad differentiering av navelsträngsblod härrör CD34+ hematopoietiska stamceller och stamceller till de fyra myeloida linjerna. Tillämpningarna av detta protokoll omfattar utredningar om effekten av myeloida sjukdoms mutationer eller små molekyler på myeloisk differentiering av CD34+ -cellerna.

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Utvecklingsbiologi CD34+ celler myeloisk differentiering hematopoietisk Stem och stamceller granulocyter monocyt erytrocyter megakaryocyt vanliga myeloisk progenitorceller granulocyt monocyt progenitorceller megakaryocyt erytroidprogenitor flödescytometri
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