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Neuroscience
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来自成人大脑的短期自由浮动切片培养
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Short-Term Free-Floating Slice Cultures from the Adult Human Brain
Please note that all translations are automatically generated.
Click here for the English version.
来自成人大脑的短期自由浮动切片培养
DOI:
10.3791/59845-v
•
09:14 min
•
November 05, 2019
•
Artur Fernandes
2
,
Niele Dias Mendes
3
,
Glaucia Maria Almeida
,
Giovanna Orlovski Nogueira
,
Carla de Moraes Machado
,
Jose de Anchieta de Castro Horta-Junior
,
João Alberto Assirati Junior
,
Norberto Garcia-Cairasco
,
Luciano Neder
,
Adriano Sebollela
1
Department of Biochemistry and Immunology, Ribeirão Preto Medical School
,
University of São Paulo
,
2
Department of Physiology, Ribeirão Preto Medical School
,
University of São Paulo
,
3
Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School
,
University of São Paulo
,
4
Department of Anatomy, Institute of Biosciences
,
São Paulo State University
,
5
Clinical Hospital at the Ribeirão Preto Medical School
,
University of São Paulo
Chapters
00:04
Title
01:14
Setting Up the Slicing Apparatus
02:14
Sample Collection
02:45
Slicing
04:14
Culture
05:18
Morphological Evaluation
06:39
Results: Analysis of the Short-Term Free-Floating Slice Cultures
08:12
Conclusion
Summary
Automatic Translation
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Automatic Translation
提出了一种从成人大脑中制备自由浮动切片培养物的协议。该协议是使用膜插入物广泛使用的切片培养方法的变体。它简单,经济高效,建议进行短期检测,旨在解开与年龄相关的脑疾病背后的神经退化机制。
Tags
Brain Slice Culture
Free-floating Slice Culture
Brain Surgery
Neurodegeneration
Cell Biology
Immunohistochemistry
Vibratome
Agarose
Slicing
Transport
Carbogen
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