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建立胃癌患者衍生异种移植模型和原细胞系
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Cancer Research
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JoVE Journal Cancer Research
Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
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建立胃癌患者衍生异种移植模型和原细胞系

DOI:
10.3791/59871-v

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08:42 min

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July 19, 2019

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, , ,
1State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine,Sun Yat-sen University Cancer Center

Chapters

  • 00:04Title
  • 01:18Establishment of PDX Model
  • 02:22Tissue Cryopreservation
  • 04:48Isolation of Primary Cells
  • 07:13Results: Analysis of Established Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

目前的协议描述了从外科胃癌样本中建立患者衍生异种移植(PDX)模型和原发性癌细胞系的方法。这些方法为药物开发和癌症生物学研究提供了有用的工具。

Tags

Patient-derived Xenograft ModelsPrimary Cell LinesTumor Biology ResearchDrug DevelopmentCancer Cell LinesNSG MiceTumor MicroenvironmentTumor DissectionTumor Cryopreservation

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