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Mekanistisk Insight inn i utviklingen av TNBS-mediert intestinal fibrose og evaluere den hemmende effekten av Rapamycin
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin
DOI:

10:21 min

September 12, 2019

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Chapters

  • 00:04Title
  • 00:52Induction of TNBS Fibrosis and Rapamycin Treatment in Mice
  • 01:58Isolation of Intestinal Lamina Propria and Purification of Cx3Cr1+ Mononuclear Phagocytes
  • 04:01Purification of Mononuclear Phagocytes from the Collected Cells Purification: Magnetic Purification
  • 04:51Purification of Mononuclear Phagocytes from the Collected Cells Purification: FACS Sorting
  • 05:39Flow Cytometry Analysis
  • 07:39Results: Analysis of TNBS-Mediated Intestinal Fibrosis
  • 09:27Conclusion

Summary

Automatic Translation

I denne studien beskriver vi en detaljert prosedyre av TNBS-mediert tarm fibrose, som utstillinger sammenlignbare patofysiologi til Crohns fibrose. Vi diskuterer også denne tilnærmingen i lys av Rapamycin tilrettelagt hemmende effekter på intestinal fibrose.

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