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Mekanistisk insikt i utvecklingen av TNBS-medierad intestinal fibros och utvärdering av de hämmande effekterna av rapamycin
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin
DOI:

10:21 min

September 12, 2019

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Chapters

  • 00:04Title
  • 00:52Induction of TNBS Fibrosis and Rapamycin Treatment in Mice
  • 01:58Isolation of Intestinal Lamina Propria and Purification of Cx3Cr1+ Mononuclear Phagocytes
  • 04:01Purification of Mononuclear Phagocytes from the Collected Cells Purification: Magnetic Purification
  • 04:51Purification of Mononuclear Phagocytes from the Collected Cells Purification: FACS Sorting
  • 05:39Flow Cytometry Analysis
  • 07:39Results: Analysis of TNBS-Mediated Intestinal Fibrosis
  • 09:27Conclusion

Summary

Automatic Translation

I denna studie beskriver vi ett detaljerat förfarande av TNBS-medierad intestinal fibros, som uppvisar jämförbar patofysiologi till Crohns fibros. Vi diskuterar också denna strategi mot bakgrund av rapamycin underlättade hämmande effekter på intestinal fibros.

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