Investigation of the Transcriptional Role of a <em>RUNX1</em> Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Chi-Keung Cheng; Terry  H.Y. Wong; Yuk-Lin Yung; Nelson  C.N. Chan; Margaret  H.L. Ng

doi:10.3791/60130

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Gransking av Transcriptional rolle en RUNX1 Intronic lyddemper av CRISPR/Cas9 Ribonucleoprotein i akutt myelogen leukemi celler

Investigation of the Transcriptional Role of a <em>RUNX1</em> Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Gransking av Transcriptional rolle en RUNX1 Intronic lyddemper av CRISPR/Cas9 Ribonucleoprotein i akutt myelogen leukemi celler

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7,591 Views

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09:16 min

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September 01, 2019

DOI:

10.3791/60130-v

DOI:

10.3791/60130-v

•

09:16 min

September 01, 2019

•

7570 Views

Chi-Keung Cheng¹, Terry H.Y. Wong¹, Yuk-Lin Yung¹, Nelson C.N. Chan¹, Margaret H.L. Ng^1,2

¹Blood Cancer Cytogenetics and Genomics Laboratory, Department of Anatomical and Cellular Pathology, Prince of Wales Hospital,The Chinese University of Hong Kong ²State Key Laboratory in Oncology in South China,The Chinese University of Hong Kong

Summary

Direkte levering av forhåndsmonterte Cas9/guide RNA ribonucleoprotein komplekser er en rask og effektiv måte for Genova redigering i blodkreft celler. Her bruker vi denne tilnærmingen til å slette en RUNX1 intronic lyddemper og undersøke transcriptional svar i OCI-AML3 Leukemic celler.