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Journal / Genetics / Gransking av Transcriptional rolle en RUNX1…
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Genetics

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JoVE Journal Genetics
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Gransking av Transcriptional rolle en RUNX1 Intronic lyddemper av CRISPR/Cas9 Ribonucleoprotein i akutt myelogen leukemi celler

Article DOI: 10.3791/60130-v • 09:16 min • September 1st, 2019
September 1st, 2019 •
1, 1, 1, 1, 1,2
1Blood Cancer Cytogenetics and Genomics Laboratory, Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, 2State Key Laboratory in Oncology in South China, The Chinese University of Hong Kong

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Direkte levering av forhåndsmonterte Cas9/guide RNA ribonucleoprotein komplekser er en rask og effektiv måte for Genova redigering i blodkreft celler. Her bruker vi denne tilnærmingen til å slette en RUNX1 intronic lyddemper og undersøke transcriptional svar i OCI-AML3 Leukemic celler.

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Genetikk CRISPR/CAS9 ribonucleoprotein electroporation CIS-regulatoriske element lyddemper Transcriptional kontroll leukemi
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