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造血干细胞代谢分析

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Developmental Biology

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JoVE Journal Developmental Biology

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

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造血干细胞代谢分析

DOI:

10.3791/60234-v

•

12:20 min

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November 09, 2019

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Giorgia Scapin^2,3, Marie C. Goulard^2,3, Priyanka R. Dharampuriya^2,3, Jennifer L. Cillis^2,3, Dhvanit I. Shah^2,3

¹Nationwide Children’s Hospital, ²The Ohio State University College of Medicine, ³The Ohio State University Comprehensive Cancer Center

Chapters

00:05Title
01:13Preparation of Reagents on the Day Prior to the Assay
02:09Day of the Assay
07:10Mitochondrial Respiration and Glycolysis Assays of LSK HSPCs
09:21Results: Analysis of Hematopoietic Stem Progenitor Cell Metabolism
11:37Conclusion

Summary

Automatic Translation

造血干细胞（HSPCs）由于血液形成过程中的代谢可塑性，从静止状态过渡到分化状态。在这里，我们提出了一个优化的方法，用于测量线粒体呼吸和HSPCs的糖解。

Tags

Hematopoietic Stem Cells Hematopoietic Progenitor Cells Metabolic Regulation Mitochondrial Respiration Glycolysis Extracellular Flux Analysis Murine Bone Marrow Density Gradient Mononucleated Cells

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