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Developmental Biology
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造血幹細胞細胞代謝の解析
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Analysis of Hematopoietic Stem Progenitor Cell Metabolism
Please note that all translations are automatically generated.
Click here for the English version.
造血幹細胞細胞代謝の解析
DOI:
10.3791/60234-v
•
12:20 min
•
November 09, 2019
•
Giorgia Scapin
2,3
,
Marie C. Goulard
2,3
,
Priyanka R. Dharampuriya
2,3
,
Jennifer L. Cillis
2,3
,
Dhvanit I. Shah
2,3
1
Nationwide Children’s Hospital
,
2
The Ohio State University College of Medicine
,
3
The Ohio State University Comprehensive Cancer Center
Chapters
00:05
Title
01:13
Preparation of Reagents on the Day Prior to the Assay
02:09
Day of the Assay
07:10
Mitochondrial Respiration and Glycolysis Assays of LSK HSPCs
09:21
Results: Analysis of Hematopoietic Stem Progenitor Cell Metabolism
11:37
Conclusion
Summary
Automatic Translation
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Automatic Translation
造血幹細胞(HSPC)は、血液形成中の代謝可塑性による静止状態から分化状態に移行します。ここでは、HSPCのミトコンドリア呼吸と糖状化を測定するための最適化された方法を提示する。
Tags
Hematopoietic Stem Cells
Hematopoietic Progenitor Cells
Metabolic Regulation
Mitochondrial Respiration
Glycolysis
Extracellular Flux Analysis
Murine Bone Marrow
Density Gradient
Mononucleated Cells
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