Journal
/
Bioengineering
/
用于初级肝细胞保存的散装滴滴 Vit;
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Bulk Droplet Vitrification for Primary Hepatocyte Preservation
Please note that all translations are automatically generated. Click here for the English version.

用于初级肝细胞保存的散装滴滴 Vit;

DOI:
10.3791/60250-v

•

11:07 min

•

October 25, 2019

•

, , , , , , , ,
1Center for Engineering in Medicine,Harvard Medical School, Massachusetts General Hospital, 2Shriners Hospitals for Children, Boston, 3Department of Surgery,University of Amsterdam

Chapters

  • 00:04Title
  • 01:01Vitrification Solutions and Apparatus Preparation
  • 03:47Hepatocyte Pre-incubation with Cryoprotective Agents (CPAs)
  • 04:59Bulk Droplet Vitrification
  • 05:51Cryogenic Storage
  • 07:00Rewarming of the Vitrified Hepatocyte Droplets
  • 09:05Results: Comparison of Bulk Droplet Vitrification to Classic Cryopreservation
  • 10:21Conclusion

Summary

Automatic Translation

Automatic Translation

本手稿描述了一种用于大量大鼠肝细胞的无冰冷冻保存方法，即原生细胞以低浓度的预孵化，并在大液滴中进行玻璃化。

Tags

Bulk Droplet VitrificationPrimary Hepatocyte PreservationCryoprotective AgentsOsmotic DehydrationCell CryopreservationHepatocyte TransplantationBio-artificial LiverBlood ProductsStem Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

荧光检测方法，为微流体液滴平台

微流体流钱伯斯再造使用血止血建模和血小板输注<em>体外</em

使用多色荧光检测液滴微流控光纤

微RNA循环使用定量DNA结合染料化学和液滴的数字PCR

微流体流室模型血小板输注与止血措施，实时血小板沉积和纤维蛋白形成

一种基于液滴的单链 dna 放大术和微球 pcr 扩增法

用于玻璃化和变暖步骤中囊胚行为客观评估的形态学协议

生物修复和益生菌发育的微生物菌株的勘探，用于生物有机物的研究与保存

高通量酵母菌株，带基于滴滴的RNA测序

使用声学分离器对CHO收获的细胞培养液进行初步澄清

Read Article