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用于生成多细胞三维球体的 CD 实验室平台
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Bioengineering
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JoVE Journal Bioengineering
Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids
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用于生成多细胞三维球体的 CD 实验室平台

DOI:
10.3791/60399-v

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10:27 min

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November 07, 2019

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, , , ,
1Department of Mechanical Engineering, Graduate School,Chung-Ang University, 2Department of Biomedical Engineering, College of Medicine,Seoul National University

Chapters

  • 00:05Title
  • 00:28Centrifugal Microfluidic-Based Spheroid (CMS) Culture Chip Fabrication
  • 02:06Cell Preparation
  • 03:10Cell Staining
  • 03:45Monoculture Spheroid Formation
  • 05:33Co-Culture Spheroid Formation: Concentric Spheroid Formation
  • 06:28Co-Culture Spheroid Formation: Janus Spheroid Formation
  • 07:33Co-Culture Spheroid Formation: Sandwich Spheroid Formation
  • 08:48Results: Representative CMS Culture Chip Spheroid Culture Analyses
  • 09:51Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了一种运动动力离心微流体装置，可以培养细胞球体。使用该装置，单细胞或多种细胞类型的球体在高重力条件下可以很容易地共同培养。

Tags

Lab-on-a-CD PlatformMulticellular Three-dimensional SpheroidsCentrifugal ForceMicrowellsPDMSPlasma CleaningCell CultureCell DetachmentCell Labeling

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