JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Immunology and Infection
/
利用基于表观遗传学的定量PCR确定免疫细胞特性和纯度
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
Please note that all translations are automatically generated.
Click here for the English version.
利用基于表观遗传学的定量PCR确定免疫细胞特性和纯度
DOI:
10.3791/60465-v
•
08:02 min
•
February 19, 2020
•
Suman K. Pradhan
,
Jerry Guzman
,
Carl Dargitz
,
Stephanie Switalski
,
Mark Landon
,
Sven Olek
,
Bjoern Samans
,
Ulrich Hoffmueler
,
Uma Lakshmipathy
1
Cell Biology, Life Sciences Solutions
,
Thermo Fisher Scientific
,
2
California Polytechnic State University (Cal Poly)
,
3
Epiontis GmbH
,
Precision for Medicine
Chapters
00:05
Introduction
00:41
Sample Preparation
01:41
Bisulfate Conversion
02:20
CD8+ T Cell and Regulatory T Cell (Treg) Assays
05:28
T Helper Cell 17 (Th17) Assay
06:37
Results: Representative Flow and Methylation Assay Data Comparisons
07:30
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们描述了一种通过使用定量PCR(qPCR)检测的表观遗传特征来确定免疫细胞特性和纯度的可靠方法。特定位点的脱甲基化作为特定细胞类型的唯一标识符,允许识别CD8+、调节或Th17 T细胞。
Tags
Immune Cell Identity
Immune Cell Purity
Epigenetic-based Quantitative PCR
Flow Cytometry
Automated Data Analysis
Hematopoietic Cell Sample
Genomic DNA Isolation
Bisulfite Conversion
DNA Purification
Paramagnetic Beads
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
免疫细胞在受伤的脊髓组织流式细胞仪定量评估:一个细胞的分离纯化方法的新颖使用
心脏功能免疫细胞的分离
活体成像的鼠标使用2 - 光子显微镜的胸腺
定量测定的免疫反应和睡眠模式<em>果蝇</em
淋巴结脂肪垫对嵌合体的淋巴结基质细胞起源研究的一代
为马科动物疱疹病毒2诊断的呼吸道液体定量PCR检测方法的开发和验证
影像性流式细胞仪对人体免疫突触的定性和定量分析
以此 T7 噬菌体的定量 PCR 研究
流式细胞术测定小鼠胸腺、胰腺干淋巴结和脾脏的调节 t 细胞亚群
果蝇、
果蝇细胞
免疫应答的评估
Read Article