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Indsamling af frosne gnaver brain regioner for downstream analyser
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Neuroscience
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JoVE Journal Neuroscience
Collection of Frozen Rodent Brain Regions for Downstream Analyses

Indsamling af frosne gnaver brain regioner for downstream analyser

DOI:
10.3791/60474-v

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07:06 min

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April 23, 2020

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, , ,
1Department of Psychological and Brain Sciences, Gill Center for Biomolecular Research,Indiana University

Chapters

  • 00:04Introduction
  • 00:46Setup and Preparation
  • 03:39Dissection
  • 04:36Results: Analysis of RNA and Protein with the Frozen Brain Dissection Method
  • 06:13Conclusion

Summary

Automatic Translation

Automatic Translation

Denne procedure beskriver indsamling af diskrete frosne hjerneregioner for at opnå protein og RNA af høj kvalitet ved hjælp af billige og almindeligt tilgængelige værktøjer.

Tags

Frozen Brain RegionsProtein And RNA AnalysisBrain DissectionBrain MatrixLiquid NitrogenIsopentaneDry IceGlass PlateForcepsRazor BladesBrain LandmarksCD-1 Wild Type Mice

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