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Journal
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Immunology and Infection
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分化人类单细胞衍生巨噬细胞中细胞外陷阱释放的体外刺激与可视化
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Immunology and Infection
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JoVE Journal
Immunology and Infection
In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
Please note that all translations are automatically generated.
Click here for the English version.
分化人类单细胞衍生巨噬细胞中细胞外陷阱释放的体外刺激与可视化
DOI:
10.3791/60541-v
•
08:08 min
•
November 01, 2019
•
Yunjia Zhang
2
,
Benjamin S. Rayner
2
,
Mathias Jensen
,
Clare L. Hawkins
2,3
1
Heart Research Institute
,
2
Sydney Medical School
,
University of Sydney
,
3
Department of Biomedical Sciences
,
University of Copenhagen
Chapters
00:05
Title
01:35
Polarization of HMDM (Human Monocyte-derived Macrophages)
02:36
Stimulation of HMDM to Induce MET (Macrophage Extracellular Traps) Release
04:20
Visualization of MET in Live Cell Culture
05:46
Results: Morphological Changes of Differentially Polarized HMDMs and Release of METs Following Stimulation
07:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是一种使用显微镜和荧光染色检测活细胞培养中巨噬细胞外陷阱 (MET) 生产的协议。此方案可进一步扩展,通过免疫荧光染色检查特定的 MET 蛋白标记物。
Tags
Extracellular Trap
Macrophages
Neutrophils
Immune Cells
Innate Immunity
Inflammatory Pathologies
In Vitro Model
Primary Human Cells
Monocyte-derived Macrophages
M1 Priming
M2 Priming
Hypochlorous Acid
PMA
TNF-alpha
IL-8 Stimulation
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