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从卡西甲抗性前列腺癌患者的甲酸固定组织和血清衍生的外源体对小型非编码RNA进行测序
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JoVE Journal Cancer Research
Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
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从卡西甲抗性前列腺癌患者的甲酸固定组织和血清衍生的外源体对小型非编码RNA进行测序

DOI:
10.3791/60549-v

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12:13 min

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November 19, 2019

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, , , ,
1Department of Biochemistry and Molecular Biology,Augusta University, 2Department of Urology,Veterans Affairs Medical Center and University of California, San Francisco, 3Department of Pathology,Veterans Affairs Medical Center and University of California, San Francisco

Chapters

  • 00:05Title
  • 01:11Isolating Serum-derived EVs (Extracellular Vesicles)
  • 02:14Library Preparation for Small RNA Sequencing: Generate Adapter-Ligated cDNA
  • 06:08Library Preparation for Small RNA Sequencing: Purify and Perform Quality Control for the Ligated cDNA
  • 09:39Results: Library Preparation, Quality Check, and Run Metrics for Sequencing Small Non-coding RNA
  • 10:50Conclusion

Summary

Automatic Translation

Automatic Translation

治疗阻力经常在晚期前列腺癌患者中发展，在某些情况下，癌症进展到一种称为神经内分泌前列腺癌的致命亚型。评估促进这种转变的微小非编码RNA介导的分子变化，将有助于更好地进行疾病分层，并识别导致神经内分泌前列腺癌发展的因果机制。

Tags

RNA SequencingSerum-derived ExosomesCastration-resistant Prostate CancerNext-generation SequencingCDNA Library PreparationRNA IsolationExosome IsolationProstate Cancer Biology

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