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Cancer Research

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Identifikation af Transskription Factor Regulators ved hjælp af Medium-Throughput Screening af Arrayed Biblioteker og en Dual-Luciferase-baserede Reporter
 

Identifikation af Transskription Factor Regulators ved hjælp af Medium-Throughput Screening af Arrayed Biblioteker og en Dual-Luciferase-baserede Reporter

Article doi: 10.3791/60582
March 27th, 2020 Usage Statistics

Summary March 27th, 2020

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For at identificere nye regulatorer af transskription faktorer, vi udviklet en tilgang til at screene arrayed lentiviral eller retroviral RNAi biblioteker ved hjælp af en dual-luciferase-baserede transskriptions-reporter assay. Denne tilgang tilbyder en hurtig og relativt billig måde at screene hundredvis af kandidater i et enkelt eksperiment.

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