JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Cancer Research
/
使用阵列库的中等通量筛选和基于双 Luciferase 的分录报告器识别转录因子稳压器
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Cancer Research
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
Please note that all translations are automatically generated.
Click here for the English version.
使用阵列库的中等通量筛选和基于双 Luciferase 的分录报告器识别转录因子稳压器
DOI:
10.3791/60582-v
•
11:32 min
•
March 27, 2020
•
Yuxuan Xiao
,
John M. Lamar
1
Department of Molecular & Cellular Physiology
,
Albany Medical College
Chapters
00:04
Introduction
00:39
Lentiviral Vector Library Preparation
01:24
Packaging of the Arrayed Lentiviral Library
03:35
Cell Infection for Screening
04:48
Cell Seeding for Transfection of Dual-Luciferase Reporter
06:27
Dual-Luciferase Reporter Transfection
07:35
Dual-Luciferase Activity Quantification
09:02
Results: Representative Arrayed Library Screening
10:47
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
为了识别新的转录因子调节器,我们开发了一种使用基于双透明酶的转录报告器测定筛选排列的慢病毒或逆转录病毒RNAi库的方法。这种方法提供了一种快速且相对便宜的方法,在单个实验中筛选数百名候选人。
Tags
Transcription Factor
Medium-throughput Screening
Arrayed Libraries
Dual-luciferase Reporter
Lentiviral Vector
Retroviral Assay
Bacterial Miniprep
293FT Cells
Transfection
PsPAX2
VSVG
Cell Culture
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
切片乳腺全身为病变鉴别
流动 Cytometry-based 药物筛选系统对促进细胞分化胶质母细胞瘤小分子的鉴定
全基因组的 rna 干扰筛查以确定调节溶瘤病毒治疗的宿主因素
腺病毒报告系统 TGF β/Smad3 信号通路的活体
细胞成像
用水凝胶微室阵列 (HMCA) 板进行肿瘤细胞侵袭和抗转移药物筛选的分析
小肠神经内分泌肿瘤类球体的建立与特征
使用转录组学分析绘制无序致癌转录因子的结构-功能关系
使用
卡诺哈布迪炎埃莱甘斯
识别 EGFR 和 RAS 抑制剂
使用快速和定量的细胞内胆碱膜模型发现转移调节器
快速筛选工作流程,利用患者衍生胶质瘤干细胞确定GBM的潜在组合疗法
Read Article