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JoVE Journal Cancer Research
Identificatie van Transcription Factor Regulators met behulp van Medium-Throughput Screening van arrayed bibliotheken en een Dual-Luciferase-Based Reporter
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Identificatie van Transcription Factor Regulators met behulp van Medium-Throughput Screening van arrayed bibliotheken en een Dual-Luciferase-Based Reporter

Article DOI: 10.3791/60582-v • 11:32 min • March 27th, 2020
March 27th, 2020 •
1, 1
1Department of Molecular & Cellular Physiology, Albany Medical College

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Om nieuwe regelgevers van transcriptiefactoren te identificeren, ontwikkelden we een benadering om arrayed lentivirale of retrovirale RNAi-bibliotheken te screenen met behulp van een dual-luciferase-gebaseerde transcriptie-verslaggever test. Deze aanpak biedt een snelle en relatief goedkope manier om honderden kandidaten in één experiment te screenen.

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Cancer Research transcriptie factor arrayed RNAi scherm YAP TAZ TEAD dual-luciferase reporter transcriptie verslaggever assay kanker
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