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In vivo augmentation af Gut-homing regulerende T-celle induktion
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Chapters
- 00:05Title
- 00:571-alpha-Hydroxylase and Retinaldehyde Dehydrogenase 2 (RALDH2) Lentivirus (Lenti-CYP-ALDH virus) Preparation
- 03:04Bone Marrow-Derived Dendritic Cell (BMDC) Generation
- 05:06BMDC Lenti-CYP-ALDH Transduction
- 06:00Results: Representative Gut-Homing Regulatory T Cell Induction
- 07:38Conclusion
Her præsenterer vi en protokol for in vivo augmentation af Gut-homing regulatoriske T-celle induktion. I denne protokol er dendritiske celler konstrueret til lokalt at producere høje koncentrationer af det aktive vitamin D (1,25-dihydroxyvitamin D eller 1,25 [OH]2D) og den aktive vitamin a (RETINSYRE eller RA) de Novo.
