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In vivo augmentation af Gut-homing regulerende T-celle induktion
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
DOI:

08:02 min

January 22, 2020

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Chapters

  • 00:05Title
  • 00:571-alpha-Hydroxylase and Retinaldehyde Dehydrogenase 2 (RALDH2) Lentivirus (Lenti-CYP-ALDH virus) Preparation
  • 03:04Bone Marrow-Derived Dendritic Cell (BMDC) Generation
  • 05:06BMDC Lenti-CYP-ALDH Transduction
  • 06:00Results: Representative Gut-Homing Regulatory T Cell Induction
  • 07:38Conclusion

Summary

Automatic Translation

Her præsenterer vi en protokol for in vivo augmentation af Gut-homing regulatoriske T-celle induktion. I denne protokol er dendritiske celler konstrueret til lokalt at producere høje koncentrationer af det aktive vitamin D (1,25-dihydroxyvitamin D eller 1,25 [OH]2D) og den aktive vitamin a (RETINSYRE eller RA) de Novo.

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