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生成受控动态化学景观,研究微生物行为
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior
DOI:

10:07 min

January 31, 2020

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Chapters

  • 00:05Introduction
  • 00:35Fabrication of the Microfluidic Device for the Single Chemical-pulse Experiment
  • 02:02Fabrication of the 3D-printed Millifluidic Device for the Experiment with Multiple Pulses
  • 05:28Multiple Chemical-pulse Experiment
  • 07:11Results: Bacterial Responses to a Chemical Pulse and Swimming Statistics in the Absence of Chemical Gradients
  • 08:50Conclusion

Summary

Automatic Translation

提出了在微流体和微流体设置中通过光液生成动态化学景观的协议。该方法适用于研究不同的生物过程,包括单细胞和种群层面的移动行为、营养摄入或对微生物化学物质的适应。

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