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Bioengineering
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生成受控动态化学景观,研究微生物行为
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior
Please note that all translations are automatically generated.
Click here for the English version.
生成受控动态化学景观,研究微生物行为
DOI:
10.3791/60589-v
•
10:07 min
•
January 31, 2020
•
Francesco Carrara
,
Douglas R. Brumley
,
Andrew M. Hein
,
Yutaka Yawata
5
,
M. Mehdi Salek
,
Kang Soo Lee
,
Elzbieta Sliwerska
,
Simon A. Levin
,
Roman Stocker
1
Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering
,
2
School of Mathematics and Statistics
,
University of Melbourne
,
3
Institute of Marine Sciences
,
University of California, Santa Cruz
,
4
Faculty of Life and Environmental Sciences
,
University of Tsukuba
,
5
Microbiology Research Center for Sustainability
,
University of Tsukuba
,
6
Department of Ecology and Evolutionary Biology
,
Princeton University
Chapters
00:05
Introduction
00:35
Fabrication of the Microfluidic Device for the Single Chemical-pulse Experiment
02:02
Fabrication of the 3D-printed Millifluidic Device for the Experiment with Multiple Pulses
05:28
Multiple Chemical-pulse Experiment
07:11
Results: Bacterial Responses to a Chemical Pulse and Swimming Statistics in the Absence of Chemical Gradients
08:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
提出了在微流体和微流体设置中通过光液生成动态化学景观的协议。该方法适用于研究不同的生物过程,包括单细胞和种群层面的移动行为、营养摄入或对微生物化学物质的适应。
Tags
Microfluidics
Chemical Gradients
Microbial Behavior
Chemotaxis
Photolysis
PDMS
Soft Lithography
3D Printing
Master Fabrication
Microenvironments
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