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将激光捕获微切片和微流体qPCR相结合,分析单细胞的转录曲线:阿片类药物依赖的系统生物学方法
JoVE Journal
Genetics
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JoVE Journal Genetics
Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence
DOI:

09:54 min

March 08, 2020

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Chapters

  • 00:05Introduction
  • 00:59Preparing the Samples
  • 02:16Laser Capture Microdissection
  • 04:07Single-cell Microfluidic RT-qPCR
  • 08:08Results: Microfluidic qPCR Yields Accurate and Anatomically Specific Transcriptional Profiles of Single-Cells
  • 09:21Conclusion

Summary

Automatic Translation

该协议解释了如何使用激光捕获微解剖,以高精度和解剖特异性从杏仁核的中央核收集单个神经元、微胶质和星形细胞。此外,我们解释我们使用微流体RT-qPCR测量这些细胞转录的子集。

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