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Kombinert bruk av hale vene metastasering analyser og Real-Time in vivo Imaging å kvantifisere brystkreft metastatisk kolonisering og byrde i lungene
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
DOI:

10:32 min

December 19, 2019

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Chapters

  • 00:04Title
  • 00:32Generation of Cancer Cells Stably Expressing Luciferase and a Fluorescent Protein
  • 01:59Tail Vein Injection of Labeled Cancer Cells
  • 04:17Monitoring the Metastatic Burden by Bioluminescence with an in vivo Live Animal Imaging Device
  • 06:18Quantification of the Number and Size of Metastases and Analysis of the Imaging Data
  • 08:01Results: YAP and TAZ are Required for Metastatic Colonization and Subsequent Growth of Breast Cancer Cells in Syngeneic Mice
  • 09:36Conclusion

Summary

Automatic Translation

Det beskrevet innfallsvinkel syndikatene eksperimentelle hale vene metastasering analyser med inne vivo bo dyr tenkelig å tillate virkelig-tid avlytting av brystkreften metastasering formasjon og oppblomstringen foruten kvantifisering av metastasering antallet og størrelse inne det lungen.

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