JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
应用活细胞成像和低温电子断层扫描解决
军团菌肺瘤
点/Icm分泌系统的时空特征
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the
Legionella pneumophila
Dot/Icm Secretion System
Please note that all translations are automatically generated.
Click here for the English version.
应用活细胞成像和低温电子断层扫描解决
军团菌肺瘤
点/Icm分泌系统的时空特征
DOI:
10.3791/60693-v
•
09:12 min
•
March 10, 2020
•
David Chetrit
,
Donghyun Park
2
,
Bo Hu
,
Jun Liu
2
,
Craig R. Roy
1
Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine
,
Yale University School of Medicine
,
2
Microbial Sciences Institute
,
Yale University
,
3
Department of Microbiology and Molecular Genetics, McGovern Medical School
,
The University of Texas Health Science Center at Houston
Chapters
00:04
Introduction
00:55
Live Cell Imaging of
L. pneumophila
with Fluorescently Tagged Dot/Icm Components
02:47
Quantification of Polar Localization and Dynamics of Dot/Icm Components
05:36
Detection of sfGFP Mass Density with Cryo-ET
06:23
Results: Imaging Dot/Icm Secretion Systems in Intact Bacteria
08:17
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
细菌细胞成像是一种新兴的系统生物学方法,侧重于定义静态和动态过程,这些过程决定了大型大分子机器的功能。在这里,定量活细胞成像和低温电子断层扫描的集成用于研究
军团菌肺瘤
IV型分泌系统架构和功能。
Tags
Live Cell Imaging
Cryo-electron Tomography
Legionella Pneumophila
Dot/Icm Secretion System
Bacterial Secretion Complexes
Host-pathogen Interactions
Structural-functional Relationships
Agarose Pads
Fluorescence Microscopy
Polarity Quantification
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
使用低温电子断层扫描和自动子断层扫描平均HIV包膜糖蛋白分子结构的测定
动态活细胞成像的宿主 - 病原体相互作用的研究使用的光学陷阱
T细胞实时成像实时信号复合物的形成
顺行运输Alphaherpes病毒传播的活细胞成像
使用<em>大蜡螟</em>作为模式生物来研究<em>嗜肺军团菌</em>感染
成像InlC的分泌由细菌病原体调查感染蜂窝<em>单核细胞增生李斯特氏菌</em
施加诱导表达系统研究与细胞内信号细菌毒力因子的干扰
嗜肺军团菌
外膜囊泡:隔离及对巨噬细胞促炎潜力分析
一种新型大鼠自然杀伤细胞无饲养系统的研究
体外
小鼠肾上腺的分离、固定和免疫荧光成像
Read Article