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February 27, 2020
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Summary
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Ce flux de travail décrit la performance de l’enrichissement rentable et temporel de multiples modifications post-traductionnelle (PTM) de protéines simultanément pour l’analyse protéomique globale quantitative. Le protocole utilise l’enrichissement ptM de niveau peptidique avec de multiples anticorps conjugués, suivi d’une analyse de spectrométrie de masse d’acquisition indépendante des données pour obtenir des connaissances biologiques sur le crosstalk PTM.
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Cite this Article
Xie, X., Shah, S., Holtz, A., Rose, J., Basisty, N., Schilling, B. Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization. J. Vis. Exp. (156), e60780, doi:10.3791/60780 (2020).
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