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Stabil knockdown af gener kodning Ekstracellulære Matrix Proteiner i C2C12 Myoblast Cell Line Ved hjælp af Small-Hairpin (sh)RNA
JoVE Journal
Genetics
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JoVE Journal Genetics
Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA
DOI:

12:19 min

February 12, 2020

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Chapters

  • 00:04Introduction
  • 00:55C2C12 Transfection
  • 02:35Transfected C2C12 Selection
  • 03:15C2C12 Differentiation
  • 03:59Phenotypic Analysis
  • 06:10Western Blot Analysis Sample Preparation
  • 09:37Results: Representative C2C12 Selection, Differentiation, and Knockdown Validation
  • 11:19Conclusion

Summary

Automatic Translation

Vi leverer en protokol til stably knockly nedslag gener kodning ekstracellulære matrix (ECM) proteiner i C2C12 myoblaster ved hjælp af små hårnål (sh) RNA. Målretning ADAMTSL2 som et eksempel, beskriver vi metoderne til validering af knockdown effektivitet på mRNA, protein, og cellulære niveau under C2C12 myoblast til myotube differentiering.

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