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Developmental Biology
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在无馈体和化学定义的培养条件下,使用人类多能干细胞的后多能感感神经元的有效分化
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Developmental Biology
Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions
Please note that all translations are automatically generated.
Click here for the English version.
在无馈体和化学定义的培养条件下,使用人类多能干细胞的后多能感感神经元的有效分化
DOI:
10.3791/60843-v
•
10:24 min
•
May 24, 2020
•
Hsueh Fu Wu
2
,
Nadja Zeltner
2,3
1
Center for Molecular Medicine
,
University of Georgia
,
2
Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences
,
University of Georgia
,
3
Department of Cellular Biology, Franklin College of Arts and Sciences
,
University of Georgia
Chapters
00:04
Introduction
00:50
Human Pluripotent Stem Cell (hPSC) Maintenance
01:48
hPSC Differentiation (Day 0)
03:34
Neural Crest Cell (NCC) Induction (Day 1-10)
04:02
NC Spheroid Aggregation (Day 10)
05:14
NC Spheroid Maintenance and Sympathetic Progenitor Induction (Day 11-14)
06:09
Sympathetic Neuron (symN) Differentiation and Maturation (Day 14-20 or 28-35)
07:38
Results: Representative NC Induction, Maintenance, and Expansion
09:43
Conclusion
Summary
Automatic Translation
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Automatic Translation
在此协议中,我们描述了一种稳定、高效的分化策略,用于从人类多能干细胞生成后强感致感神经元。此模型将使神经元可用于多个自主障碍的研究。
Tags
Human Pluripotent Stem Cells
Sympathetic Neurons
Feeder-free
Chemically Defined
Efficient Differentiation
Electrical Activity
Disease Modeling
Regenerative Medicine
Drug Screening
Co-culture System
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