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Journal / Genetics / Convalida del sequenziamento dei nanopore…
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Genetics

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JoVE Journal Genetics
Convalida del sequenziamento dei nanopore dell'intero genoma, usando Usutu Virus come esempio
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Convalida del sequenziamento dei nanopore dell'intero genoma, usando Usutu Virus come esempio

Article DOI: 10.3791/60906-v • 05:45 min • March 11th, 2020
March 11th, 2020 •
1, 1, 1, 1
1ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research, Erasmus University Medical Center

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In precedenza abbiamo convalidato un protocollo per il sequenziamento del virus USsutu intero genoma basato sull'amplicon (USUV) su una piattaforma di sequenziamento nanoporo. Qui, descriviamo i metodi utilizzati in modo più dettagliato e determiniamo il tasso di errore della cella di flusso nanopora R10.

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Genetica Numero 157 nanoporo sequenziamento cella a flusso R10 USUV arbovirus sequenziamento dell'intero genoma
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