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Journal
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Developmental Biology
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使用接近连接测定在
C.elegans
Germline中对核糖蛋白复合体装配的实地检测
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
In Situ Detection of Ribonucleoprotein Complex Assembly in the
C. elegans
Germline using Proximity Ligation Assay
Please note that all translations are automatically generated.
Click here for the English version.
使用接近连接测定在
C.elegans
Germline中对核糖蛋白复合体装配的实地检测
DOI:
10.3791/60982-v
•
08:56 min
•
May 05, 2020
•
Nicholas J. Day
,
Xiaobo Wang
,
Ekaterina Voronina
1
Division of Biological Sciences
,
University of Montana
Chapters
00:04
Introduction
00:49
Dissection/Gonad Extrusion
02:46
Fixation/Blocking
04:14
Primary Antibody Incubation and PLA Probe (Secondary Antibody) Incubation
05:21
Ligation
06:09
Amplification and Final Washes
06:45
Coverslip Mounting
07:15
Results: Germlines Following Co-immunostaining or PLA
08:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议演示了使用接近连接测定来探测
在C. elegans
生殖系
中原位
的蛋白质-蛋白质相互作用。
Tags
Proximity Ligation Assay
Protein-protein Interactions
C. Elegans
Germline
Dissection
Immunofluorescence
Microscopy
Article
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