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The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
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JoVE Journal Developmental Biology
The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

DOI:
10.3791/55113-v

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07:34 min

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February 16, 2017

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, ,
1Department of Biological Sciences,Mississippi State University

Chapters

  • 00:05Title
  • 00:51Microinjection of Morpholino Oligonucleotides
  • 02:31Fixation
  • 03:40Hybridization
  • 04:54In Situ Development
  • 05:40Slide Preparation and Image Capture
  • 06:22Results: Identification of Molecules Involved in Wnt Anterior-posterior Specification
  • 07:01Conclusion

Summary

Automatic Translation

Automatic Translation

This video article details a straightforward in vivo methodology that can be used to systematically and efficiently characterize components of complex signaling pathways and regulatory networks in many invertebrate embryos.

Tags

Keywords: Sea Urchin EmbryosIn Vivo Developmental ModelsCell-to-cell Signaling NetworksGene Regulatory NetworksSignaling PathwaysCell Fate SpecificationEvolutionary And Developmental BiologySignal Transduction PathwaysFunctional AnalysisNext-generation Differential ScreensGenomic And Morphological Simplicity

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