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Cancer Research
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斑马鱼原发性患者衍生肿瘤异种移植物的药物筛选
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Cancer Research
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JoVE Journal
Cancer Research
Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish
Please note that all translations are automatically generated.
Click here for the English version.
斑马鱼原发性患者衍生肿瘤异种移植物的药物筛选
DOI:
10.3791/60996-v
•
09:50 min
•
April 10, 2020
•
Meghan G. Haney
2
,
L. Henry Moore
,
Jessica S. Blackburn
2
1
Department of Molecular and Cellular Biochemistry
,
University of Kentucky
,
2
Markey Cancer Center
,
University of Kentucky
Chapters
00:04
Introduction
00:54
Fluorescently Labeling Cells with DiI(1,1 -Dioctadecyl-3,3,3 ,3 -Tetramethylindocarbocyanine Perchlorate)
02:10
Microinjecting Zebrafish Larvae
04:37
Setting up Drug Screen with Xenografted Zebrafish
06:22
Imaging Xenografted Zebrafish and Quantifying Fluorescence
08:36
Results: Xenografted Tumor Area
09:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
斑马鱼异种移植模型允许在体内微环境中对人类癌细胞进行高通量药物筛选和荧光成像。我们使用配备自动化荧光显微镜的成像单元,为斑马鱼患者衍生白血病样本进行大规模的自动化药物筛查工作流程。
Tags
Zebrafish Xenograft
Drug Screening
Primary Patient-derived Tumor
Automated Imaging
Quantification
Human Cancer Cells
Drug Response
In Vivo Replicant
Cost-effective
Dii Staining
Cell Resuspension
Agarose Plate
Zebrafish Dechlorination
Microinjection
Borosilicate Glass Needle
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