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Journal
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Bioengineering
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高通量酵母菌株,带基于滴滴的RNA测序
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
高通量酵母菌株,带基于滴滴的RNA测序
DOI:
10.3791/61014-v
•
07:55 min
•
May 21, 2020
•
Jesse Q. Zhang
2
,
Kai-Chun Chang
,
Leqian Liu
,
Zev J. Gartner
5
,
Adam R. Abate
4,5
1
Department of Bioengineering and Therapeutic Sciences
,
University of California San Francisco
,
2
University of California Berkeley-UCSF Graduate Program in Bioengineering
,
University of California San Francisco
,
3
Department of Pharmaceutical Chemistry
,
University of California San Francisco
,
4
California Institute for Quantitative Biosciences
,
University of California San Francisco
,
5
Chan Zuckerberg Biohub
Chapters
00:04
Introduction
00:29
Yeast Encapsulation in Hydrogels Using Device A
01:39
Breaking and Washing Hydrogels
02:58
Yeast Colony Lysis
04:04
mRNA Capture Using Device B
05:27
Results: High-Throughput Screening of
C. albicans
07:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
微生物工程"设计-构建-测试"周期的一个瓶颈是,我们可以执行应变功能屏幕的速度。我们描述了一种高通量方法,用于菌株筛选,应用于每个实验的数百至数千个酵母细胞,利用基于滴滴的RNA测序。
Tags
High Throughput
Yeast Strain
Phenotyping
Droplet-based RNA Sequencing
ICO-seq
Microbes
Genome-wide
Engineered Microbes
Agarose
PBS
Syringe Pump
Fluorinated Oil
Fluorosurfactant
Emulsion
Perfluoro-octanol
Tet(W) Buffer
Yeast Culture Medium
Hydrogels
Spheroplasting
Article
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