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为铁氧化还原物种(Fe(II)、Fe(III)定量设置毛细管电泳-电感耦合等离子体质谱仪(CE-ICP-MS)
JoVE Journal
Chemistry
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JoVE Journal
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Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))
Please note that all translations are automatically generated.
Click here for the English version.
为铁氧化还原物种(Fe(II)、Fe(III)定量设置毛细管电泳-电感耦合等离子体质谱仪(CE-ICP-MS)
DOI:
10.3791/61055-v
•
04:48 min
•
May 04, 2020
•
Bernhard Michalke
,
Desiree Willkommen
4
,
Vivek Venkataramani
3
1
Research Unit Analytical BioGeoChemistry
,
Helmholz Zentrum München-German Research Center for Environmental Health
,
2
Department of Hematology and Medical Oncology
,
University Medical Center Göttingen (UMG)
,
3
Institute of Pathology
,
University Medical Center Göttingen (UMG)
,
4
RECIPE Chemicals + Instruments GmbH
Chapters
00:04
Introduction
00:43
CE-ICP-MS Interface Setup
03:01
Results: Analysis of SH-SY5Y Cell Lysate
03:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
这种铁氧化氧光谱法基于毛细管电泳-电感耦合等离子质谱法,样品堆叠与短分析相结合。该方法可快速分析铁氧化还原物种的定量限制,用于各种组织和生物流体样品。
Tags
CE-ICP-MS
Iron Redox Species
Fe(II)
Fe(III)
Quantification
Nebulizer
Interface
Capillary
Luer Connectors
Silicone Tube
Platinum Wire
Calibration Curves
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