JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Chemistry
/
使用硫代巴比妥酸反应物质测定法评估生物样品中的氧化应激
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Chemistry
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Please note that all translations are automatically generated.
Click here for the English version.
使用硫代巴比妥酸反应物质测定法评估生物样品中的氧化应激
DOI:
10.3791/61122-v
•
06:19 min
•
May 12, 2020
•
Jesús Aguilar Diaz De Leon
,
Chad R. Borges*
1
School of Molecular Sciences, The Biodesign Institute – Center for Personalized Diagnostics
,
Arizona State University
Chapters
00:04
Introduction
01:08
Reagent Preparation
02:26
TBARS Assay
03:56
Results: Thiobarbituric Acid Reactive Substances Assay
05:30
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
硫代巴比妥酸反应物质测定的目的是通过使用可见光波长分光光度法在532nm处测量脂质过氧化产物(主要是丙二醛)的产生来评估生物样品中的氧化应激。这里描述的方法可应用于人血清、细胞裂解物和低密度脂蛋白。
Tags
TBARS Assay
Oxidative Stress
Biological Samples
MDA
Thiobarbituric Acid
Sodium Hydroxide
Sodium Acetate
Spectrophotometric Analysis
Sample Preparation
Standard Curve
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
不相关的代谢组学,生物源使用超高效液相色谱 - 高分辨质谱(UPLC-HRMS)
抗癌金属配合物的合成与细胞毒性评价MTT法
超临界氮处理的活性多孔材料的净化
使用聚苯乙烯<em>块</em> - 聚(丙烯酸)涂层的金属纳米颗粒作为单体他们均聚物和共聚物聚合
铱氧化物纳米颗粒对硒化镉硫化镉@纳米棒光化学氧化增长
土壤样品多涨价可能分析的 MPLEx 协议
用亚硫酸氢萃取协议分离混合物中的醛和反应性酮
基于丝绸的自行式微搅拌器的反应式喷墨打印及推进分析
硫醇反应试剂的合成与生物共轭作用--用于原位选择性修饰免疫共轭的生成
[
11
C_SNAP-7941] 自动合成和实时动力学评估的技术方面
Read Article