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Isolering af endokardie- og koronarenendotelceller fra den ventrikelfrie frie væg af rottehjertet
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

Isolering af endokardie- og koronarenendotelceller fra den ventrikelfrie frie væg af rottehjertet

DOI:
10.3791/61126-v

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08:22 min

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April 15, 2020

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, , ,
1Department of Pediatrics, Division of Cardiology,Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease,Stanford School of Medicine, 3Stanford Cardiovascular Institute,Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute,Cincinnati Children’s Hospital Medical Center

Chapters

  • 00:04Introduction
  • 01:11Heart Isolation
  • 02:34Endocardial Endothelial Cell (EEC) Digestion
  • 03:34Coronary Endothelial Cell (CEC) Digestion
  • 04:36Endothelial Cell (EC) Sorting
  • 06:56Results: Representative qPCR Data CEC and EEC Isolation Verification
  • 07:46Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer en protokol for isolering af endokardie- og koronarenendotelceller fra rottehjerter gennem sekventiel vævsfordøjelse i en fordøjelsesbuffer, cellesamling fra tilbagevendende centrifugecyklusser og cellerensning ved hjælp af anti-rotte CD31 mikroperler.

Tags

Endocardial CellsCoronary Endothelial CellsHeartIsolationCell Type-specificGene ExpressionHeart DiseasesTranscriptomicsEpigeneticsSprague Dawley RatsVentricular Free WallDissectionDigestion Buffer

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