Journal
/
Bioengineering
/
Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy

Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy

DOI:
10.3791/61154-v

•

08:01 min

•

May 12, 2020

•

, , , , ,
1Pennsylvania Muscle Institute, Perelman School of Medicine,University of Pennsylvania, 2Department of Physics,West Chester University

Chapters

  • 00:04Introduction
  • 00:31Glass Coverslip Cleaning
  • 01:36Evaporative Deposition of Polystyrene Beads
  • 03:22Bead Annealing for Reducing Pore Size
  • 03:55Nanofabrication of Aluminum Zero Mode Waveguides
  • 05:42Results: ZMW Fabrication Quality Control
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

Described here is a nanosphere lithography method for parallel fabrication of zero mode waveguides, which are arrays of nanoapertures in a metal-clad glass microscopy coverslip for single molecule imaging at nano- to micromolar concentrations of fluorophores. The method takes advantage of colloidal crystal self-assembly to create a waveguide template.

Tags

Zero Mode WaveguidesSingle Molecule MicroscopyNanosphere LithographyEvaporative DepositionPolystyrene BeadsHumidity ChamberOptical Borosilicate Glass CoverslipsAcetonePotassium HydroxideEthanolCentrifugationRelative Humidity

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition

In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses

Read Article