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Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
JoVE Journal
Biology
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JoVE Journal Biology
Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

DOI:
10.3791/61239-v

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08:24 min

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May 20, 2020

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, , ,
1Center for Veterinary Medicine,U.S. Food and Drug Administration

Chapters

  • 00:04Introduction
  • 00:49Preparation of DNA Templates
  • 02:25Assembly of a LAMP Reaction
  • 03:50LAMP Result Interpretation
  • 05:49Representative Results: Salmonella Sensitivity Panel
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

Loop-mediated isothermal amplification (LAMP) is an isothermal nucleic acid amplification test (iNAAT) that has attracted broad interest in the pathogen detection field. Here, we present a multi-laboratory-validated Salmonella LAMP protocol as a rapid, reliable, and robust method for screening Salmonella in animal food and confirming presumptive Salmonella from culture isolation.

Tags

Keywords: LAMPSalmonellaAnimal FoodScreeningConfirmationRapid TestingPCRDNA TemplatesSample PreparationIsothermal AmplificationCross-contamination

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