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Journal
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Biochemistry
/
膜蛋白質相互作用解析用のネイティブ細胞膜ナノ粒子システム
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Biochemistry
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JoVE Journal
Biochemistry
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
Please note that all translations are automatically generated.
Click here for the English version.
膜蛋白質相互作用解析用のネイティブ細胞膜ナノ粒子システム
DOI:
10.3791/61298-v
•
07:31 min
•
July 16, 2020
•
Kyle G. Kroeck
2
,
Weihua Qiu
2
,
Claudio Catalano
2
,
Thi Kim Hoang Trinh
2
,
Youzhong Guo
2
1
Department of Medicinal Chemistry, School of Pharmacy
,
Virginia Commonwealth University
,
2
Institute for Structural Biology, Drug Discovery and Development, School of Pharmacy
,
Virginia Commonwealth University
Chapters
00:05
Introduction
00:54
Native Cell Membrane Nanoparticle (NCMN) Preparation
03:04
Negative Stain Grid Preparation
04:55
Results: Representative Negative Stain and Electrophoresis Gel images of Purified Acriflavine Resistance B (AcrB) Constructs
06:52
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここで提示される電子顕微鏡法と組み合わせてネイティブ細胞膜ナノ粒子系を利用する膜蛋白質のオリゴマー状態の決定のためのプロトコルです。
Tags
Native Cell Membrane Nanoparticles
Membrane Protein-protein Interaction Analysis
Electron Microscopy
Membrane Protein Structure
Native Environment
High-resolution Structure Determination
Membrane Active Polymer
Nickel NTA Column
Fast Protein Liquid Chromatography
Negative Stain Grids
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