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测量一组啮齿动物脑样本中的中风后脑水肿、梗塞区和血脑屏障破裂

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

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测量一组啮齿动物脑样本中的中风后脑水肿、梗塞区和血脑屏障破裂

DOI:

10.3791/61309-v

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04:32 min

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October 23, 2020

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Dmitry Frank*¹, Benjamin F. Gruenbaum*², Julia Grinshpun, Israel Melamed, Olena Severynovska, Ruslan Kuts, Michael Semyonov, Evgeni Brotfain, Alexander Zlotnik, Matthew Boyko

¹Department of Anesthesiology and Critical Care, Soroka Medical Center,Ben-Gurion University of the Negev, ²Department of Anesthesiology,Yale University School of Medicine, ³Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences,Ben-Gurion University of the Negev, ⁴Department of Physiology, Faculty of Biology, Ecology and Medicine,Dnepropetrovsk State University

Chapters

00:04Introduction
00:54Determination of Infarct Zone and Brain Edema
02:37Determination of BBB Disruption
03:23Results: Histological Outcomes of MCAO Rats Compared to Sham-operated Rats
03:57Conclusion

Summary

Automatic Translation

此协议描述了在同一组啮齿动物脑样本上测量缺血性脑损伤三个最重要的参数的新技术。仅使用一个大脑样本在道德和经济成本方面非常有利。

Tags

Keywords: Stroke Cerebral Edema Infarct Zone Blood-brain Barrier BBB Permeability Middle Cerebral Artery Occlusion Ischemic Brain Injury TTC Staining ImageJ Infarct Volume Brain Edema Trichloroacetic Acid Luminescence Spectrophotometry

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