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Chapters
- 00:05Introduction
- 00:43Oothecae Collection
- 01:34Oothecae Cleaning
- 02:04Oothecae Sterilization and Incubation
- 03:47Gnotobiotic Nymph Maintenance
- 04:44Results: Representative Oothecae Characterization and Growth Analyses
- 05:31Conclusion
This protocol is used in establishing and maintaining gnotobiotic American cockroaches (Periplaneta americana) by surface sterilizing the egg cases (oothecae) prior to hatching. These gnotobiotic insects contain their vertically transmitted Blattabacterium endosymbionts but have axenic guts.
