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En model af Epileptogenese i Rhinal Cortex-Hippocampus Organotypic Slice Cultures
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures

En model af Epileptogenese i Rhinal Cortex-Hippocampus Organotypic Slice Cultures

DOI:
10.3791/61330-v

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10:05 min

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March 18, 2021

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, , ,
1Instituto de Farmacologia e Neurociências,Faculdade de Medicina da Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes,Faculdade de Medicina da Universidade de Lisboa

Chapters

  • 00:04Introduction
  • 00:49Rhinal Cortex-Hippocampus Slice Preparation
  • 03:41Electrophysiological Recordings
  • 05:07Propidium Iodide (PI) Uptake Assay and Immunohistochemistry
  • 07:26Results: Epileptic-like Events, Neuronal Death and Gliosis are Observed in Rhinal Cortex-Hippocampus Organotypic Slices
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi forberedelsen af rhinal cortex-hippocampus organotypic skiver. Under en gradvis og kontrolleret afsavn af serum skildrer disse skiver udviklende epileptiske-lignende begivenheder og kan betragtes som en ex vivo-model af epileptogenese. Dette system repræsenterer et glimrende værktøj til overvågning af dynamikken i spontan aktivitet samt til vurdering af udviklingen af neuroinflammatoriske træk i løbet af epileptogenese.

Tags

Keywords: EpilepsyEpileptogenesisRhinal CortexHippocampusOrganotypic Slice CulturesEx Vivo ModelSprague-DawleyGBSSOlfactory BulbsHippocampusDentate GyrusCA AreasEntorhinal CortexPerirhinal CortexCulture MediumCell Culture Incubator

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