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Journal / Neuroscience / Optogenetische activering van afferente paden in…
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JoVE Journal Neuroscience
Optogenetische activering van afferente paden in hersenplakken en modulatie van reacties door vluchtige anesthetica
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Optogenetische activering van afferente paden in hersenplakken en modulatie van reacties door vluchtige anesthetica

Article DOI: 10.3791/61333-v • 08:16 min • July 23rd, 2020
July 23rd, 2020 •
1, 1,2, 1, 1
1Department of Anesthesiology, University of Wisconsin, 2Department of Anesthesiology, Rambam Health Care Campus, the Ruth and Bruce Rappaport Faculty of Medicine, Technion - Israel Institute of Technology

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Ex vivo hersenplakken kunnen worden gebruikt om de effecten van vluchtige anesthetica op opgeroepen reacties op afferente inputs te bestuderen. Optogenetica wordt gebruikt om zelfstandig thalamocorticale en corticocorticale afferente stoffen te activeren voor niet-primaire neocortex, en synaptische en netwerkresponsen worden gemoduleerd met isofluraan.

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Neurowetenschappen Nummer 161 channelrhodopsinen optogenetica thalamus neocortex isofluraan anesthesie inhalatie elektrofysiologie patch-clamp technieken interneuronen
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