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Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice
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JoVE Journal Neuroscience
Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice

Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice

DOI:
10.3791/61377-v

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08:58 min

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July 02, 2020

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1Department of Biology, Neurobiology, and Bio-X,Stanford University

Chapters

  • 00:04Introduction
  • 01:13Setup
  • 02:33Transcardial Perfusion and Brain Extraction
  • 04:57Slicing and Recovery
  • 06:24Results: Patch-clamp and Synaptic Plasticity Measurements in Hippocampal Slices from Mice at P120 180
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

This is a protocol for acute slice preparation from adult and aging mouse hippocampi that takes advantage of transcardial perfusion and slice cutting with ice-cold NMDG-aCSF to reduce hypoxic damage to the tissue. The resulting slices stay healthy over many hours, and are suitable for long-term patch-clamp and field-recordings.

Tags

Hippocampal SlicesAdultAgingHypoxiaHypothermiaNMDG-aCSFTranscardial PerfusionVibrating MicrotomeField-recordingsPatch-clampNeurodegenerative Diseases

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