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Journal
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Biochemistry
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用于植物微RNA检测和定量的RNA斑点分析
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
Please note that all translations are automatically generated.
Click here for the English version.
用于植物微RNA检测和定量的RNA斑点分析
DOI:
10.3791/61394-v
•
14:41 min
•
July 11, 2020
•
Varsha Tirumalai
2
,
Melvin Prasad
,
P. V. Shivaprasad
1
National Centre for Biological Sciences
,
Tata Institute of Fundamental Research
,
2
SASTRA University
Chapters
00:00
Introduction
01:27
Step 1: Preparation of a 15% Denaturing Polyacrylamide Gel
02:03
Step 2: Assembly of the Glass Plates and Electrophresis Unit
04:13
Step 3: Preparation of Loading Dye and Samples
05:38
Step 4: Gel Electrophresis
06:33
Step 5: Preparation for Electro-blotting
10:15
Step 6: Preparation of Radiolabeled Probe
10:50
Step 7: Hybridization of the Blot
12:36
Representative Results
13:24
Conclusion
Summary
Automatic Translation
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Automatic Translation
此方法演示了利用北方杂交技术从总RNA提取物中检测miRNA。
Tags
RNA Blot Analysis
MicroRNA Detection
Small RNA Northern Blot
Acrylamide Gel Electrophoresis
RNA Quantification
Precursor MicroRNA Detection
Modified Northern Protocol
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