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Journal / Immunology and Infection / 髄膜炎菌…
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Immunology and Infection

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JoVE Journal Immunology and Infection
髄膜炎菌 人工多能性幹細胞由来脳内皮細胞の感染
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
Journal (Immunology and Infection)
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis
Journal (Immunology and Infection)
A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis
 
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髄膜炎菌 人工多能性幹細胞由来脳内皮細胞の感染

Article DOI: 10.3791/61400-v • 10:12 min • July 14th, 2020
July 14th, 2020 •
1, 2, 1, 1,2
1Institute for Hygiene and Microbiology, University of Würzburg, 2Department of Biological Sciences, University of Alabama

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ここに記述されているプロトコルは伝染のための Neisseriaの髄膜炎 の準備、および他の分子分析のためのサンプル収集を分化させる誘導された多能性幹細胞によって得られる脳そっくりのendothelialセル、準備の主要なステップを強調する。

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髄膜炎菌、髄膜炎菌性髄膜炎、脳内皮細胞、宿主-病原体相互作用、生体内モデルシステム、ヒトベースモデル、タイトジャンクション、経内皮電気抵抗(TEER)、In Vitroモデル、一次BEC、不死化BEC、人工多能性幹細胞(iPS細胞)、IPSC由来BEC(iPSC-BEC)、バリア破壊、自然免疫活性化、細菌間相互作用
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