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Chemistry
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直接耦合电雷电图(DC-ERG),用于记录小鼠视网膜颜料皮皮的光唤起电响应
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Chemistry
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JoVE Journal
Chemistry
Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
Please note that all translations are automatically generated.
Click here for the English version.
直接耦合电雷电图(DC-ERG),用于记录小鼠视网膜颜料皮皮的光唤起电响应
DOI:
10.3791/61491-v
•
09:10 min
•
July 14, 2020
•
Kiyoharu J. Miyagishima
,
Congxiao Zhang*
2
,
Volha V. Malechka*
3
,
Kapil Bharti
,
Wei Li
1
Retinal Neurophysiology Section, National Eye Institute
,
National Institutes of Health
,
2
Ocular and Stem Cell Translational Research Section, National Eye Institute
,
National Institutes of Health
,
3
Human Visual Function Core, National Eye Institute
,
National Institutes of Health
Chapters
00:04
Introduction
00:44
Capillary Electrode Filling
01:51
Microelectrode Holder Preparation
02:46
Electrode Test
04:08
Mouse and Electrode Positioning
06:15
Direct-Coupled Electroretinogram (DC-ERG) Recording
07:04
Results: Representative DC-ERG Traces and Workflow Analyses
08:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一种方法,使用马莫斯坦、佩奇和同事在21世纪早期首次描述的一种称为DC-ERG的技术,记录小鼠视网膜色素上皮(RPE)的光唤起电反应。
Tags
Direct-coupled Electroretinogram
DC-ERG
Retinal Pigment Epithelium
Non-invasive Evaluation
Age-related Changes
Disease Progression
Pharmacological Intervention
Reproducibility
Capillary Electrode
HBSS
Vacuum Chamber
Custom Stand
Microelectrode Holder
T-clip
Magnetic Ball Joint
Electrode Test
Ground Electrode
Reference Electrode
Article
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